{"id":1091,"date":"2025-10-29T23:00:00","date_gmt":"2025-10-29T23:00:00","guid":{"rendered":"http:\/\/sebigec.es\/blog\/?guid=c8473c50ce8f521935d67be410bf4d28"},"modified":"2025-10-29T23:00:00","modified_gmt":"2025-10-29T23:00:00","slug":"a-targeted-lc-ms-ms-based-quantitative-assay-for-detecting-plasma-factor-xiii-a-b-subunit-deficiency","status":"publish","type":"post","link":"https:\/\/sebigec.es\/blog\/index.php\/2025\/10\/29\/a-targeted-lc-ms-ms-based-quantitative-assay-for-detecting-plasma-factor-xiii-a-b-subunit-deficiency\/","title":{"rendered":"A Targeted LC-MS\/MS\u2013Based Quantitative Assay for Detecting Plasma Factor XIII A\/B Subunit Deficiency"},"content":{"rendered":"Factor XIII (FXIII) is a heterotetramer that plays a crucial role in the coagulation cascade, facilitating fibrin crosslinking to stabilize clots. Congenital or acquired deficiency of the FXIII A or B subunits can lead to prolonged bleeding. Existing enzyme-linked immunosorbent assay\u2013based and latex agglutination assays for measuring FXIII are limited to detecting only the FXIIIA subunit of the complex. In this study, a targeted liquid chromatography\u2013tandem mass spectrometry (LC-MS\/MS) assay was developed and validated to accurately quantify both FXIIIA and FXIIIB subunits in plasma.","protected":false},"excerpt":{"rendered":"<p>Factor XIII (FXIII) is a heterotetramer that plays a crucial role in the coagulation cascade, facilitating fibrin crosslinking to stabilize clots. Congenital or acquired deficiency of the FXIII A or B subunits can lead to prolonged bleeding. Existing e&#8230;<\/p>\n","protected":false},"author":0,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6,54],"tags":[55],"class_list":["post-1091","post","type-post","status-publish","format-standard","hentry","category-articulos","category-the-journal-of-molecular-diagnostics","tag-jmoldiagn"],"_links":{"self":[{"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/posts\/1091","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/types\/post"}],"replies":[{"embeddable":true,"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/comments?post=1091"}],"version-history":[{"count":25,"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/posts\/1091\/revisions"}],"predecessor-version":[{"id":1092,"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/posts\/1091\/revisions\/1092"}],"wp:attachment":[{"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/media?parent=1091"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/categories?post=1091"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/tags?post=1091"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}