{"id":10301,"date":"2026-02-25T14:00:00","date_gmt":"2026-02-25T14:00:00","guid":{"rendered":"http:\/\/sebigec.es\/blog\/?guid=a770986bd92b58a4fb7c873ab1902282"},"modified":"2026-02-25T23:00:00","modified_gmt":"2026-02-25T23:00:00","slug":"droplet-digital-and-reverse-transcription-quantitative-pcr-comparison-for-circulating-mir-371a-3p-in-malignant-germ-cell-tumors","status":"publish","type":"post","link":"https:\/\/sebigec.es\/blog\/index.php\/2026\/02\/25\/droplet-digital-and-reverse-transcription-quantitative-pcr-comparison-for-circulating-mir-371a-3p-in-malignant-germ-cell-tumors\/","title":{"rendered":"Droplet-Digital and Reverse Transcription Quantitative PCR Comparison for Circulating miR-371a-3p in Malignant Germ Cell Tumors"},"content":{"rendered":"Previous work has shown that circulating miR-371a-3p has higher sensitivity and specificity than current biomarkers for malignant germ cell tumors (MGCT). Here, the performance of two methods commonly used to measure miR-371a-3p levels was compared: reverse transcription quantitative PCR (RT-qPCR) and droplet-digital PCR (ddPCR). Samples from the University of Texas Southwestern (UTSW) Medical Center and the University of Cambridge (UoC) were evaluated using both RT-qPCR and ddPCR, as per current protocols (RT-qPCR) or standard manufacturer\u2019s recommendations (ddPCR).","protected":false},"excerpt":{"rendered":"<p>Previous work has shown that circulating miR-371a-3p has higher sensitivity and specificity than current biomarkers for malignant germ cell tumors (MGCT). Here, the performance of two methods commonly used to measure miR-371a-3p levels was compared: re&#8230;<\/p>\n","protected":false},"author":0,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6,54],"tags":[55],"class_list":["post-10301","post","type-post","status-publish","format-standard","hentry","category-articulos","category-the-journal-of-molecular-diagnostics","tag-jmoldiagn"],"_links":{"self":[{"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/posts\/10301","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/types\/post"}],"replies":[{"embeddable":true,"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/comments?post=10301"}],"version-history":[{"count":25,"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/posts\/10301\/revisions"}],"predecessor-version":[{"id":10547,"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/posts\/10301\/revisions\/10547"}],"wp:attachment":[{"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/media?parent=10301"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/categories?post=10301"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sebigec.es\/blog\/index.php\/wp-json\/wp\/v2\/tags?post=10301"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}