Highly Sensitive Detection of Donor Chimerism by Next-Generation Sequencing

Donor chimerism analysis is used for monitoring engraftment status and risk of disease relapse following allogeneic stem cell transplantation. Recently developed assays using next generation sequencing (NGS) have demonstrated enhanced sensitivity and accuracy compared to standard capillary electrophoresis methods. We report here our validation results using One Lambda Devyser Chimerism assay, an NGS-based test for monitoring donor chimerism. A total of 270 samples, including clinical and cell line DNA, were tested.