Enhanced Detection of EGFRvIII in Tumors: A Comparative Study of Split Read and Read Depth-Based DNA Sequencing Approaches

Epidermal Growth Factor Receptor variant III (EGFRvIII), a common oncogenic variant in glioblastoma (GBM) and other solid tumors, results from an in-frame deletion of exons 2–7 in the EGFR gene. Detection of EGFRvIII is crucial for understanding tumor biology, guiding targeted therapies, and development of personalized treatment strategies. In this study, two detection approaches based on DNA next-generation sequencing (NGS)—read depth (RD)-based and split read (SR)-based detection—were compared to evaluate their sensitivity and accuracy in identifying EGFRvIII.