A Laboratory-Adaptive Dynamic Quality Control Framework Reduces Targeted Capture Sequencing Failure in Solid Tumors by >90%

High failure rates in targeted capture sequencing of solid tumors—especially from formalin-fixed, paraffin-embedded samples—limit the clinical application of next-generation sequencing. Current wet-laboratory quality control (QC) relies on rigid, predefined thresholds, which are not adaptable to the heterogeneity of clinical samples and contribute significantly to sequencing failures. Retrospective analysis of QC parameters from 1146 tumor samples (425-gene panel; 2021 to 2023) identified five independent predictors of failure: total DNA amount, nucleic acid quality, DNA input, prelibrary total DNA, and prelibrary input (all P < 0.05).